Analytics Core Facility Essen
Organization
The Analytics Core Facility Essen (ACE) is a central facility of the Center of Medical Biotechnology (ZMB) and of the Faculty of Biology of the University Duisburg-Essen. It offers access to state-of-the-art mass spectrometry-based proteomics for internal and external users. The timely technical equipment of the ACE allows us to provide a wide variety of different “bottom-up” and “top-down” proteomics methods. Furthermore, we also develop innovative solutions for project-specific demands. The service comprises all aspects of mass spectrometry-based proteomics, starting from technical advice and project planning, sample preparation and analysis to data analysis, data management and interpretation of results. We are also listed in the RIsources Poral of the DFG (RI_00466).
Contact
Address
Analytics Core Facility Essen (ACE)
Universität Duisburg-Essen
ZMB
Universitätsstr. 2
45141 Essen
ACE Core Facility Manager
Dr. Farnusch Kaschani
office: S03 S02 A62
MS-Labs: T03 R-1 C11/C14/C18
office: +49201 183 4924
MS-Lab: +49201 183 6839
Publications
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The CAPE1 peptide confers resistance against bacterial wilt in tomatoIn: Journal of Experimental Botany (JEB) 2025 , in pressDOI (Open Access)
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An unusual glycerol-3-phosphate dehydrogenase in Sulfolobus acidocaldarius elucidates the diversity of glycerol metabolism across ArchaeaIn: Communications Biology , Vol. 8 2025, Nr. 1, 539DOI (Open Access)
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Sulphostin-inspired N-phosphonopiperidones as selective covalent DPP8 and DPP9 inhibitorsIn: Nature Communications , Vol. 16 2025, Nr. 1, 3208DOI (Open Access)
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Lack of AtMC1 catalytic activity triggers autoimmunity dependent on NLR stabilityIn: EMBO Reports 2025 , in pressDOI (Open Access)
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The Actin‐Binding Prolyl‐Isomerase Par17 Sustains Its Substrate Selectivity by Interdomain AllosteryIn: Proteins: Structure, Function and Genetics 2025 , in pressDOI (Open Access)
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Subtilase SBT5.2 inactivates flagellin immunogenicity in the plant apoplastIn: Nature Communications , Vol. 15 2024, Nr. 1, 10431DOI (Open Access)
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Characterization of 2-phenanthroate:CoA ligase from the sulfate-reducing, phenanthrene-degrading enrichment culture TRIPIn: Applied and Environmental Microbiology , Vol. 90 2024, Nr. 10, e0129624DOI (Open Access)
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Environmental activity-based protein profiling for function-driven enzyme discovery from natural communitiesIn: Environmental Microbiomes , Vol. 19 2024, Nr. 1, 36DOI, Online Full Text (Open Access)
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Extracellular plant subtilases dampen cold-shock peptide elicitor levelsIn: Nature Plants , Vol. 10 2024, Nr. 11, pp. 1749 – 1760DOI (Open Access)
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The anti-tubercular callyaerins target the Mycobacterium tuberculosis-specific non-essential membrane protein Rv2113In: Cell Chemical Biology , Vol. 31 2024, Nr. 10, pp. 1755 – 1771.e73DOI (Open Access)
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Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in plantaIn: Scientific Reports , Vol. 14 2024, Nr. 1, 10091DOI (Open Access)
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Factors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHsIn: mSystems , Vol. 9 2024, Nr. 9, e0042224DOI (Open Access)
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High resolution analysis of proteolytic substrate processingIn: The Journal of Biological Chemistry (JBC) , Vol. 300 2024, Nr. 11, 107812DOI (Open Access)
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Induced degradation of SNAP-fusion proteinsIn: RSC Chemical Biology , Vol. 5 2024, Nr. 12, pp. 1232 – 1247DOI (Open Access)
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The proteome of Nicotiana benthamiana is shaped by extensive protein processingIn: New Phytologist , Vol. 243 2024, Nr. 3, pp. 1034 – 1049DOI (Open Access)
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Microtubule end-on attachment maturation regulates Mps1 association with its kinetochore receptorIn: Current Biology , Vol. 34 2024, Nr. 11, pp. 2279 – 2293.e6DOI (Open Access)
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TopBP1 utilises a bipartite GINS binding mode to support genome replicationIn: Nature Communications , Vol. 15 2024, Nr. 1, 1797DOI, Online Full Text (Open Access)
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Activity-based proteomics uncovers suppressed hydrolases and a neo-functionalised antibacterial enzyme at the plant–pathogen interfaceIn: New Phytologist , Vol. 241 2024, Nr. 1, pp. 394 – 408DOI (Open Access)
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Differences in the Synovial Fluid Proteome of Septic and Aseptic Implant FailureIn: Antibiotics , Vol. 13 2024, Nr. 4, 346DOI, Online Full Text (Open Access)
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Glutathione Transferase Photoaffinity Labeling Displays GST Induction by Safeners and Pathogen InfectionIn: Plant and Cell Physiology , Vol. 65 2024, Nr. 1, pp. 128 – 141DOI (Open Access)
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Clp-targeting BacPROTACs impair mycobacterial proteostasis and survivalIn: Cell , Vol. 186 2023, Nr. 10, pp. 2176 – 2192.e22DOI (Open Access)
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Structure of the peroxisomal Pex1/Pex6 ATPase complex bound to a substrateIn: Nature Communications , Vol. 14 2023, Nr. 1, 5942DOI (Open Access)
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The UBX domain in UBXD1 organizes ubiquitin binding at the C-terminus of the VCP/p97 AAA-ATPaseIn: Nature Communications , Vol. 14 2023, Nr. 1, 3258DOI, Online Full Text (Open Access)
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IFNα primes cancer cells for Fusicoccin-induced cell death via 14-3-3 PPI stabilizationIn: Cell Chemical Biology , Vol. 30 2023, Nr. 6, pp. 573 – 590.e6DOI (Open Access)
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Chemoproteomics Reveals the Pan-HER Kinase Inhibitor Neratinib To Target an Arabidopsis Epoxide Hydrolase Related to Phytohormone SignalingIn: ACS Chemical Biology , Vol. 18 2023, Nr. 5, pp. 1076 – 1088
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Taspase1 facilitates Topoisomerase IIβ-mediated DNA double-strand breaks driving estrogen-induced transcriptionIn: Cells , Vol. 2023 2023, Nr. 12, 363DOI, Online Full Text (Open Access)
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The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infectionIn: Frontiers in Plant Science (FPLS) , Vol. 14 2023, 1140101DOI (Open Access)
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Erratum: Author Correction: Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profiling (Communications biology (2022) 5 1 (1254))In: Communications Biology , Vol. 5 2022, Nr. 1, pp. 1319DOI (Open Access)
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Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profilingIn: Communications Biology , Vol. 5 2022, Nr. 1, 1254DOI, Online Full Text (Open Access)
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Activity-Based Protein Profiling for the Identification of Novel Carbohydrate-Active Enzymes Involved in Xylan Degradation in the Hyperthermophilic Euryarchaeon Thermococcus sp. Strain 2319x1EIn: Frontiers in Microbiology , Vol. 12 2022, 734039DOI, Online Full Text (Open Access)
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Persister state-directed transitioning and vulnerability in melanoma
32. Deutscher Hautkrebskongress (ADO-Jahrestagung), 14.–17. September 2022, Hannover, Germany,In: Nature Communications , Vol. 13 2022, Nr. 1, pp. 35 – 36DOI, Online Full Text (Open Access) -
Article leaf apoplast of field-grown potato analyzed by quantitative proteomics and activity-based protein profilingIn: International Journal of Molecular Sciences (IJMS) , Vol. 22 2021, Nr. 21, 12033DOI (Open Access)
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Targeted substrate loop insertion by VCP/p97 during PP1 complex disassemblyIn: Nature Structural & Molecular Biology , Vol. 28 2021, Nr. 12, pp. 964 – 971
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Correction to : Natural brominated phenoxyphenols kill persistent and biofilm-incorporated cells of MRSA and other pathogenic bacteriaIn: Applied Microbiology and Biotechnology , Vol. 105 2021, Nr. 14-15, pp. 6119 – 6119DOI (Open Access)
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Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasisIn: Cell Reports , Vol. 37 2021, Nr. 8, 110056DOI, Online Full Text (Open Access)
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The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilasesIn: The Plant Journal , Vol. 106 2021, Nr. 6, pp. 1523 – 1540DOI (Open Access)
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Protein Phosphatase-1 Complex Disassembly by p97 is Initiated through Multivalent Recognition of Catalytic and Regulatory Subunits by the p97 SEP-domain AdaptersIn: Journal of Molecular Biology (JMB) , Vol. 432 2020, Nr. 23, pp. 6061 – 6074
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Accelerated trypsin autolysis by affinity polymer templatesIn: RSC Advances , Vol. 10 2020, Nr. 48, pp. 28711 – 28719DOI (Open Access)
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Accelerated trypsin autolysis by affinity polymer templatesIn: RSC Advances , Vol. 10 2020, Nr. 48, pp. 28711 – 28719DOI (Open Access)
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Natural brominated phenoxyphenols kill persistent and biofilm-incorporated cells of MRSA and other pathogenic bacteriaIn: Applied Microbiology and Biotechnology , Vol. 104 2020, Nr. 13, pp. 5985 – 5998DOI (Open Access)
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Targeting of parvulin interactors by diazirine mediated cross-linking discloses a cellular role of human Par14/17 in actin polymerizationIn: Biological Chemistry , Vol. 401 2020, Nr. 8, pp. 955 – 968DOI (Open Access)
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Discovering the RNA-binding proteome of plant leaves with an improved RNA interactome capture methodIn: Biomolecules , Vol. 10 2020, Nr. 4, 661DOI (Open Access)
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Zelkovamycin is an OXPHOS Inhibitory Member of the Argyrin Natural Product FamilyIn: Chemistry - A European Journal , Vol. 26 2020, Nr. 39, pp. 8524 – 8531DOI (Open Access)
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Using proteomics for an insight into the performance of activated sludge in a lab-scale WWTPIn: International Biodeterioration & Biodegradation , Vol. 149 2020, pp. 104934
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Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor S/CYS8 in Nicotiana benthamianaIn: Plant Biotechnology Journal , Vol. 17 2019, Nr. 8, pp. 1670 – 1678DOI (Open Access)
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Cathepsin L regulates metabolic networks controlling rapid cell growth and proliferationIn: Molecular and Cellular Proteomics (MCP) , Vol. 18 2019, Nr. 7, pp. 1330 – 1344DOI (Open Access)
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Plant chemical genetics reveals colistin sulphate as a SA and NPR1-independent PR1 inducer functioning via a p38-like kinase pathwayIn: Scientific Reports , Vol. 9 2019, Nr. 1, pp. 11196DOI (Open Access)
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Cathepsins Drive Anti-Inflammatory Activity by Regulating Autophagy and Mitochondrial Dynamics in Macrophage Foam CellsIn: Cellular Physiology and Biochemistry , Vol. 53 2019, Nr. 3, pp. 550 – 572DOI (Open Access)
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A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamianaIn: BMC Genomics , Vol. 20 2019, Nr. 1, pp. 722DOI (Open Access)
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Proteases underground : Analysis of the maize root apoplast identifies organ specific papain-like cysteine protease activityIn: Frontiers in Plant Science (FPLS) , Vol. 10 2019DOI (Open Access)
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Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptidesIn: Science , Vol. 364 2019, Nr. 6436, eaav0748DOI (Open Access)
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Triazine probes target ascorbate peroxidases in plantsIn: Plant Physiology , Vol. 180 2019, Nr. 4, pp. 1848 – 1859DOI (Open Access)
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Metabolic reconstruction of the genome of candidate Desulfatiglans TRIP_1 and identification of key candidate enzymes for anaerobic phenanthrene degradationIn: Environmental Microbiology , Vol. 21 2019, Nr. 4, pp. 1267 – 1286DOI (Open Access)
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Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seedsIn: Biochemical Journal , Vol. 476 2019, Nr. 5, pp. 843 – 857DOI (Open Access)